Olga D. Ermolaeva, Sergy A. Lukyanov, and Eugene D. Sverdlov
A novel theory of subtractive hybridization including (or based on) the kinetic model of this process was proposed. A computer program modeling the process of subtraction was developed. Basing on the theory, a novel method of subtractive hybridization was proposed allowing routine comparison of genomes and products of genome expression. The method was applied to studies of the genetic mechanisms of embryogenesis, regeneration, cell differentiation and tumor transformation.